We extend telomeres to extend the human healthspan
Short telomeres play a major role in causing the major diseases of aging including cancer, hypertension, and diabetes. Telomeres are repetitive DNA sequences that protect the ends of chromosomes but shorten over time due partly to incomplete DNA replication and oxidative damage. When telomeres become too short they are unable to protect chromosome ends, causing chromosomes to fuse and mutate, and cells to either die, senesce, or become cancerous. We have developed a method to rapidly extend telomeres to avoid these outcomes and keep people healthy longer.
TeloEX extends telomeres rapidly and transiently
TeloEX is nucleoside-modified mRNA encoding telomerase reverse transcriptase (TERT). Delivery of TeloEX to cells transiently increases telomerase activity (1-2 days), during which telomeres are rapidly extended. After the brief treatment, the extended telomeres resume shortening at their normal rate, important for safety. TeloEX extends telomeres in days by an amount sufficient to reverse over a decade of telomere shortening in vivo. TeloEX treatment increases proliferative capacity of cells in a dose-dependent manner.
Rapid telomere extension
TeloEX extends telomeres in human fibroblasts by 0.9 kb in less than a week. Controls are untreated; vehicle only; and catalytically-inactive TERT mRNA. (Ramunas, 2015).
Dose-dependent increase in cell replicative capacity
Treatment of human fibroblasts with TeloEX increases replicative capacity in a dose-dependent manner.
TeloEX has several advantages for telomere extension:
Fast: One day of TeloEX treatment reverses more than a decade of telomere shortening.
Transient: TeloEX is degraded in 1-2 days, leaving telomeres extended, but not extending.
Non-immortalizing: After treatment, extended telomeres resume shortening at their normal rate.
Hypoimmunogenic: Made of natural RNA.
Dose-dependent: TeloEX provides cells with a finite, tunable increase in proliferative capacity without immortalization.
TeloEX-based telomere extension is useful for:
Clonal expansion of genetically-modified cells (e.g. CRISPR)
Ramunas, J., Yakubov, E., Brady, J. J., Corbel, S. Y., Holbrook, C., Brandt, M, Stein, J., Santiago, J.G., Cooke, J.P. and Blau, H.M. (2015). Transient delivery of modified mRNA encoding TERT rapidly extends telomeres in human cells. The FASEB Journal 29(5):1930-1939 PMC4415018.
Rejuvenation Technologies Inc. (RTI) was founded by researchers at Stanford University passionate about extending the healthspan. We are the co-inventors of TERT mRNA-based telomere extension. We welcome inquiries of all kinds. Please email us at firstname.lastname@example.org.